> I have a project where i want to detect and count bubbles in a test tube. > I am using opencv; i used hough circle detecting algorithm. But its of no > use for this purpose > Any one know any kind of algorithm to count specific objects or any shape > analyzing techniques? > any kind of information or ideas are welcomed > thanks in advance Did you ask Gargoyle? What did it say? Some of these may be useful: http://prola.aps.org/abstract/PR/v102/i6/p1653_1 Bubble counting using an inverse acoustic scattering method http://www.umiacs.umd.edu/~ramani/pubs/bubcount.pdf Ultrasonic bubble counting technique for neutron dose measurements in bubble damage detectors This looks interesting. Method may be able to be adduced from photo or web search. Photosynthesis (bubble-counting-method) (with the Cobra3 Ba-sic-Unit) http://www.phywe.com/461/pid/26802 Interesting. Abstract but gives good outline. Acoustic bubble counting technique using sound speed extracted from sound attenuation http://ieeexplore.ieee.org/Xplore/login.jsp?reload=true&url=http://ieeexplore.ieee.org/iel5/48/19839/00917945.pdf%3Farnumber%3D917945&authDecision=-203 Photoelectric Bubble counter based on photoelectric technique for leakage detection of cryogenic valves http://www.springerlink.com/content/u343546588p65012/ R -- http://www.piclist.com PIC/SX FAQ & list archive View/change your membership options at http://mailman.mit.edu/mailman/listinfo/piclist